Search Result

Select

Surveillance and analysis of key human intestinal helminth infections in Yongxing County， Hunan Province， China

ZHU Han-wu, YANG Hui, HE De-biao, HUANG Jun-xiao

Abstract （24）

HTML

PDF （473KB）（151）

Objective To investigate the prevalence of key human intestinal helminth infections in Yongxing County, Hunan Province, China, so as to provide a scientific basis for parasitic disease prevention and control. Methods An administrative village each from five townships (located in the eastern, western, southern, northern, and central regions, respectively) was randomly selected in Yongxing County, Hunan Province from 2016 to 2020. At least 200 permanent residents ≥3 years old were surveyed at each surveillance point every year. The intestinal worm eggs were examined by using the Kato-Katz technique. The hookworm species was identified by using filter paper culture. The pinworm eggs were examined by using the anal swab method with a transparent tape for children aged 3 to 9 years. WPS Excel 2021 software was used to establish the database, and SPSS 20.0 software was used to conduct statistical analysis. The infection rate was compared using the Chi-squared test. Results A total of 5 066 persons were examined for worms in feces, with a rate of key intestinal worm infections of 2.68% (136 cases). The infection rates for roundworms, hookworms, pinworms, whipworms, and liver flukes were 1.99%, 0.24%, 0.20%, 0.18%, and 0.08%, respectively. No co-infection was detected. The results of 12 hookworm larvae culture samples were all Necator americanus. The anal swab test detected a pinworm infection rate of 0.33% among a total of 1 500 children aged 3 to 9 years. The overall key intestinal worm infection rates from 2016 to 2020 were 4.08%, 1.97%, 3.24%, 1.10%, and 3.00%, respectively. Conclusion The key human intestinal worm infection rate is at a low epidemic level in Yongxing County, Hunan Province, but the infection factors still exist. It is necessary to strengthen categorical surveillance and promotion and education of parasitic disease prevention and control knowledge.

2024, 35 (1): 85-88. doi: 10.11853/j.issn.1003.8280.2024.01.015

Select

Serological diagnosis and molecular epidemiological analysis of rickettsiosis in Lu’an， Anhui Province， China

TANG Fu-gen, LYU Yong, CHEN Zhi-chao, YANG Wei, YANG Hui, CHANG Hong-wei

Abstract （54）

HTML

PDF （3933KB）（176）

Objective From May 2017 to November 2021, there was an increased number of cases of fever with rashes admitted to Huoshan County Hospital in Lu’an City, Anhui Province, China. The aim of the study is to find out the etiology of the patients through epidemiological and clinical characteristic analysis, so as to provide a scientific basis for the prevention and control of the febrile illness with rashes. Methods An epidemiological investigation was carried out on the cases of fever with rashes through inquiring their medical histories and reviewing their medical records. The acute sera at admission and convalescent sera were collected from the patients to examine IgG antibody titers to Anaplasma, Borrelia burgdorferi, Orientia, Ehrlichia, Rickettsia rickettsii, and R. typhi using indirect immunofluorescence assay. An infection of a pathogen was determined by a 4-fold increase of the serum IgG antibody titer to the pathogen between the acute serum and the convalescent serum. The etiologic agent causing the outbreak was further identified by PCR amplification and DNA sequences of the rickettsial genes from acute serum. Results The IgG antibodies to Anaplasma, B. burgdorferi, and R. typhi were all negative in the acute sera of the patients. The positive rate of serum IgG antibodies to R. rickettsii was 51.52% (68/132) in the acute sera and 89.19% (33/37) in the convalescent sera. In the paired sera, the IgG titer had more than four time increase in the acute sera than in the convalescent sera. Phylogenetic analysis with the Rickettsia ompA sequence indicated that the etiologic agent formed a monoclade with newly identified Candidatus R. jingxinensis in Yunnan, China. Conclusion The febrile cases with rashes in Lu’an were serologically diagnosed as spotted fever rickettsial infection.

2024, 35 (1): 42-48. doi: 10.11853/j.issn.1003.8280.2024.01.008

Select

Select

Select

Select

Select

An analysis of transferrin gene expression in resistant population and susceptible population of Culex pipiens quinquefasciatus

YANG Hui-na, LU Jing, TAN Wen-bin

Abstract （269）

PDF （4151KB）（1047）

Objective To analyze the expression level of transferrin gene in the susceptible population and resistant population of Culex pipiens quinquefasciatus. Methods RNA was extracted and was amplified by quantitative real-time PCR (qRT-PCR) to produce partial gene fragments of the target gene using the designed primers. After purification and sequencing, the sequence alignment was performed. The t test was used to statistically analyze the expression levels of transferrin gene in the susceptible population and resistant population of Cx. pipiens quinquefasciatus. Results The qRT-PCR analysis showed that the mean fluorescence quantification values of transferring gene in susceptible population and resistant population of Cx. pipiens quinquefasciatus were 15.21 and 18.41, respectively; the mean fluorescence quantification values of the internal reference gene in the two populations were 20.53 and 19.10, respectively. The expression level of transferrin gene in the resistant population of Cx. pipiens quinquefasciatus was reduced, and there was a significant difference in the expression level of transferrin gene between the two populations ( t=10.390, P<0.001). Conclusion The transferrin gene of Cx. pipiens quinquefasciatus can be used as a target gene for the detection and treatment of mosquito resistance.

2019, 30 (1): 50-55. doi: 10.11853/j.issn.1003.8280.2019.01.011

Select

Study on the epidemic situation of mosquitoes populations and mosquito-borne diseases in Central Command Region of CPLA

TANG Tian, YANG Hui, DENG Bing, DU Zhi-hui

Abstract （291）

PDF （441KB）（776）

By summarizing the literature, we studied the population composition and seasonal variations of the mosquito populations in the Central Command Region of CPLA, and summed up the connection of the local epidemic diseases and mosquito population distribution. This paper summarized the mosquito population investigation and natural epidemic disease in Beijing, Tianjin, Henan, Hebei, Shanxi, Shaanxi, and Hubei provinces for the past years, and provided reference for the health and disease prevention in the Central Command Region of CPLA.

2018, 29 (6): 666-668. doi: 10.11853/j.issn.1003.8280.2018.06.031

Select

Observation on ecological characteristics of Culex pipiens pallens

YANG Hui, DENG Bing, WANG Mei-lin, YANG Hui-suo, DU Zhi-hui, ZHAO Hong-mei

Abstract （626）

PDF （504KB）（1065）

Objective To observe the blood-feeding and oviposition behaviors of Culex pipiens pallens associated with the reproductive characteristics and to evaluate the vectorial capacity. Methods Observing the adult male and female ratio of different eclosion time, studying the relation between post-mating and blood-feeding of Cx. pipiens pallens and the influence of different oviposition substrate on the mosquito's egg laying behavior. Results The mosquitoes emerged on the first day were almost all males. There were two blood-feeding peaks for females post-eclosion. The blood feeding frequency of non-mated females was higher than the mated females. The feeding frequency of delayed mated females declined. The black color and the water that raised the larvae can attract the gravid females to oviposit. Conclusion The methods to interrupt mating are not suitable for controlling the Cx. pipiens pallens. The black color and the nutritious water could be used to the mosquito's traps.

2016, 27 (5): 487-490. doi: 10.11853/j.issn.1003.8280.2016.05.017

Select

The establishment and initial application of real-time fluorescent PCR detection of Nam Dinh virus

LIANG Ke-feng, LIU Qu, WANG De-quan, ZHOU Jian-ming, JIN Yu-juan, CHEN Ying-jian, LI Jing-mei, GAN Li-ping, YANG Hui

Abstract （332）

PDF （693KB）（958）

Objective To establish a rapid and specific real-time fluorescent PCR method for Nam Dinh virus (NdiV). Methods According to the sequence alignment of GenBank and NDiV isolated by our laboratory, we found out the conservative sequence (RdRp) and design specific primers and TaqMan-MGB probe. In order to evaluate the reaction system, the concentration of primers and probe were adjusted to optimize the reaction conditions, and the sensitivity, specificity, stability tests for our method were also conducted. Results TaqMan-MGB real-time fluorescence PCR detection method of NDiV were less time-consuming and highly sensitive, and the low detection limit was 0.1 PFU. It had a good specificity characteristic for having no cross reaction with dengue serotype 1-4, epidemic encephalitis b virus, respiratory syncytial virus, rotavirus, stellate virus, and adenovirus; The five times repeated testing of four nucleic acid content in different standard samples revealed that the average coefficient of variation range of Ct was 1.67%-3.68%, and thus it had high stability. Through monitoring, the positive probability of mosquitoes collected from Longgang district for NDiV was 18.00%. Conclusion NDiV TaqMan-MGB real-time fluorescent PCR method is a rapid, specific, sensitive and stable method, it can be applied to epidemiological monitoring in order to improve the ability of rapid detection of viruses.

2015, 26 (5): 447-450. doi: 10.11853/j.issn.1003.4692.2015.05.004

Select

Survey and analysis on rodents at the ports region of Sino-Vietnam in Guangxi Zhuang Autonomous Region, China

GUO Tian-yu, LIANG Zhong-ping, LIU Li-juan, WAN Dao-zheng, YE Hai-bo, DUAN Xiao-xiao, YANG Ming-xue, YANG Hui-min, YU Jian-guo

Abstract （328）

PDF （329KB）（870）

Objective To fully clarify the species of the rodents at the port region of Sino-Vietnam in Guangxi Zhuang Autonomous Region, so as to provide basic data for the management of rodents. Methods Night trapping method and night cage method were used to collect the rodents. Results Collected were 498 rodents including 4 species at Dongxing port, 756 rodents including 14 species at Pingxiang port, 75 rodents including 6 species at Shuikou port and 173 rodents including 8 species at Longbang port. Among these rodents, Rattus norvegicus was the predominant species. Conclusion Rattus norvegicus was the key specie of the surveillance and control at the ports.

2015, 26 (3): 320-321. doi: 10.11853/j.issn.1003.4692.2015.03.027

Select

Development of rat skull automatic identification system basedon random forests

HUA Hui-zhen, YANG Hui-yong, YUAN Xiong-feng, ZOU Bo, WANG Deng, GAO Ling-wang

Abstract （318）

PDF （2312KB）（864）

Objective Rodents can survive in any existing habitat, and are the largest order of mammals in terms of both variety and quantity of species. Unlike other mammals, their classification can be variable and complex, due to the large number of species. Furthermore, the inter-species characteristics and traits tend to be convergent. They pose a seriously increasing threat to agriculture. Hence it is crucial to find effective measures to manage and control rodent infestation, which requires confirmation of their taxonomic statuses precisely and quickly. However, an expert on one species or family may be unfamiliar with another. These issues have increased the demand for digitized software tools that can recognize and characterize rodent skulls from images. In this study we developed a system, named “Rodents Skull Automatic Identification System”, based on random forests. Methods The training module and recognition module of the system were designed based on the recognition technology of computer science, OpenCV, and random forests. The training module included image input, image preprocessing, feature extraction, pattern recognition, and identification result return. The recognition module included image input, image preprocessing, feature extraction, training classifier, and classifier data storage. The system identified rodents of 13 species from 4 families through automatic extraction and analysis of 32 mathematical morphological features on dorsal maxillary images, such as eccentricity and compactness. Results The system could identify 13 species among 4 families of rodents. The results showed that the average identification accuracy rate was above 80%, and the identification accuracy rate of each species was above 70%, indicating that the system was highly reliable in recognition of rodents. Conclusion Rodents can be automatically identified with the aid of computer technology. However, this system is only a preliminary study, and it requires further studies to improve the recognition rate.

2014, 25 (5): 416-420. doi: 10.11853/j.issn.1003.4692.2014.05.008

Select

Epidemiological characteristics and prevention strategy of Japanese encephalitis from 2001 to 2010 in Shijiazhuang

GUO Jian-hua, ZHANG Shi-yong, QI Shun-xiang, YIN Zun-dong, YANG Hui-min

Abstract （846）

PDF （1345KB）（791）

Objective To provided evidence for the development of Japanese encephalitis (JE) control and prevention strategies by analysis of the epidemiological characteristics and the relationship between JE and mosquitoes. Methods During 2001-2010, JE cases were collected from the passive surveillance. At the same time, the acute encephalitis and meningitis symptom surveillance were conducted to identify JE cases from the perspective observational study during 2007-2010. Participating facilities included one children hospital, three provincial general hospitals and three rural county-level general hospitals in five county districts with random assignment. ELISA was used to detect the agent in blood and cerebrospinal fluid (CSF). The mosquito specimens were collected with labor hour method from May to October. Results There were 75 JE cases reported in Shijiazhuang. The annual average incidence rate and the fatality rate were 0.08/105 and 0.26%, respectively. Two peaks in age distribution were observed: one with children of 5-15 years, and the other with adults of 40 years and older. The inhabitant to floating population ratio was 2.57∶1. The time distribution was mainly in August, September and October. Culex pipiens pallens and Cx. tritaeniorhynchus were the dominant species. Correlation was found between JE epidemic and mosquitoes density (r_s＝0.5506, P＝0.000). The ratio of JE diagnosed was 28.95% among the first visiting patients. Conclusion JE epidemiological characteristics are changing with a gradually increasing incidence and increasing cases among floating population. It is important to enhance epidemiology and pathogen surveillance with acute encephalitis and meningitis for JE prevention and control. The preventive strategy for JE should be a comprehensive system based on vaccines immunization.

2012, 23 (1): 54-56.

Select

Investigation of mosquito populations and evaluation of control effects in Yuebing village, Shahe, Beijing

YANG Hui, MA Jing, JI Yu-chuan, LI Sen-lin, MA Tao

Abstract （1420）

PDF （912KB）（842）

Objective To identify the mosquito populations and evaluate the control effects in Yuebing village and the surrounding areas of Shahe, Beijing. Methods Mosquitoes were captured using CO₂ light traps and subject to taxonomic identification. Comprehensive control measures based on environmental management and chemical approaches were employed to kill mosquitoes. Results From March to October 2009, 8351 adult mosquitoes were captured, of which Culex pipiens pallens, Anopheles sinensis, Aedes albopictus and Cx. tritaeniorhynchus accounted for 99.57%, 0.19%, 0.18% and 0.06%, respectively. Cx. pipiens pallens was the predominant species. The mosquitoes emerged in early May, peaked in June and July, and generally disappeared by the end of September, mainly dwelling in woods and weeds surrounding the village. However, the mosquito density was relatively low in the village. The comprehensive control measures were simple and effective against mosquitoes. Conclusion The predominant species, seasonal dynamics and geographic distribution of mosquitoes in Yuebing village as well as the potential hazards were basically identified, which was of guiding value for anti-epidemic strategies in large-scale military activities in this region and for mosquito prevention and control in large-scale military assembly.

2010, 21 (4): 371-372.

Select

Role of V?shaped river groove in the elimination of mosquito, mouse and oncomelania breeding sites

KE Chun-Rong, YANG Hui, CHEN Bai-Yun, LI Hong-Jun, ZHOU Shi-Sheng, DUAN Bing-Hua

Abstract （842）

PDF （235KB）（934）

2009, 20 (5): 487-.

Select

Field investigation on efficacy of CO ₂ trapping lamp and mosquito-curtain trap catching mosquitoes

DENG Bing*; LI Chun-xiao; YANG Hui; DONG Yan-de; FU Qiang; MA Jing; ZHANG Ying-mei; LI Hong; HUANG Qing-zhen; JIA Lin; ZHAO Tong-yan

Abstract （1170）

PDF （329KB）（961）

Objective To compare the efficacy of two traps catching mosquitoes.Methods The methods of CO ₂ trapping lamp and mosquito-curtain trap were used in this study.Results There were the same mosquitoes species captured by two kinds of traps.There were 1548 mosquitoes,and Culex pipiens pallens, Aedes albopictus and Armigeres subalbatus accounted for 87.86%,11.95% and 0.19% respectively.The mosquito density index of CO ₂ trapping lamp catching mosquitoes was 21.8/lamp·hour,which was lower than that of mosquito-curtain trap(84.0/curtain·hour).Conclusion The CO ₂ trapping lamp could be used to monitor the mosquito population,especially it was the preferred method in the epidemic area of mosquito-borne disease.

Select

Effect of Juvenile hormone and Moulting hormone on biosynthesis of aggregation pheromone of the German cockroach, Blattella germanica(L.)

YANG Hui; ZHANG Jin-tong; DENG Bing; LI Hong

Abstract （1127）

PDF （220KB）（1422）

Objective To study the effect of Juvenile hormone(JH) and Moulting hormone(MH) on biosynthesis of aggregation pheromone of the German cockroach, Blattella germanica(L.) to provide information for German cockroach control. Methods The aggregation pheromone was extracted from epidermis and analysed by gas chromato-graphy(GC). Results Allatectomy in the first half stage of nymph of 5 ^th instar decreased the amount of dimethylnonacosane and methyltriacontane by 10% and 21% respectively. Injected with JHⅢ, the amount of these two compounds were increased by 32% and 24%. In the last half stage of nymph of 5 ^th instar, after extirpating prothorax the amount of the two compounds were decreased by 18% and 12%. Injected with 20-hydroxyecdysone, the amount were increased by 26% and 36% respectively. Conclusion JH and MH regulate biosynthesis of the aggregation pheromone of the B.germanica in the first half stage and the last half stage of nymph of 5 ^th instar respectively.

Select

Epidemiological Analysis on the Hemorrhagic Fever with Renal Syndrome in Taian City,1984-2001

XUE Jia-qin;YANG Hui-li;CHEN Yong;et al

Abstract （1371）

PDF （120KB）（641）

Objective Epidemiological changes of hemorrhagic fever with renal syndrome(HFRS)in Taian city were to find out.Methods Morbidity rate of HFRS among different seasons,age groups,sex,occupations from 1984 to 2001 were compared respectively.The condition of the host animal carring HFRS virus,the general population's inapparent infection and the type of HFRS epidemic foci were investigated simultaneously.Results There exited period prevalent of HFRS in Taian,and the peak morbidity rate came out per 10 years about.The incidence of HFRS in spring was higher than other seasons.The constitution of the rodent species was miscellaneous, Rattus norvegicus was the dominant in house,but the dominant in field was Apodemus agrarius.The inapparent infectious rate in epidemic areas was higher than no-epidemic areas.Conclusion Among population of 20-49 years old,men and farmers were main resource of HFRS cases,and HFRS risk of the youngsters would increased.The main source of infection was Rattus norvegicus the pathogen was type Ⅱ HFRS virus.Furthermore the endemic areas was wide-ranging and steady.